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Applied and Environmental Microbiology, March 2007, p. 2029-2032, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02320-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Multiplex PCR Assay for Detection of Vibrio vulnificus Biotype 2 and Simultaneous Discrimination of Serovar E Strains{triangledown}

Eva Sanjuán and Carmen Amaro*

Departamento Microbiología y Ecología, Universidad de Valencia, Avenida Dr. Moliner 50, 46100 Burjassot, Valencia, Spain

Received 2 October 2006/ Accepted 20 January 2007

In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.

* Corresponding author. Mailing address: Departamento Microbiología y Ecología, Universidad de Valencia, Avenida Dr. Moliner 50, 46100 Burjassot, Valencia, Spain. Phone: 34963543104. Fax: 34963544570. E-mail: carmen.amaro{at}uv.es.

{triangledown} Published ahead of print on 2 February 2007.

Applied and Environmental Microbiology, March 2007, p. 2029-2032, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02320-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sanjuan, E., Fouz, B., Oliver, J. D., Amaro, C. (2009). Evaluation of Genotypic and Phenotypic Methods To Distinguish Clinical from Environmental Vibrio vulnificus Strains. Appl. Environ. Microbiol. 75: 1604-1613 [Abstract] [Full Text]  
  • Valiente, E., Jimenez, N., Merino, S., Tomas, J. M., Amaro, C. (2008). Vibrio vulnificus Biotype 2 Serovar E gne but Not galE Is Essential for Lipopolysaccharide Biosynthesis and Virulence. Infect. Immun. 76: 1628-1638 [Abstract] [Full Text]  


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