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Applied and Environmental Microbiology, April 2007, p. 2354-2359, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02603-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Simultaneous Detection of Pseudomonas fragi, P. lundensis, and P. putida from Meat by Use of a Multiplex PCR Assay Targeting the carA Gene{triangledown}

Danilo Ercolini,* Federica Russo, Giuseppe Blaiotta, Olimpia Pepe, Gianluigi Mauriello, and Francesco Villani

Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici (NA), Italy

Received 8 November 2006/ Accepted 27 January 2007

Species-specific primers and a multiplex PCR assay were developed for the simultaneous identification and differentiation of Pseudomonas fragi, P. lundensis, and P. putida based on the coamplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (carA). The carA multiplex PCR was used to detect the presence of the three Pseudomonas species from beef, chicken, and pork samples and proved to be effective in showing their evolution during the storage of meat.

* Corresponding author. Mailing address: Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici (NA), Italy. Phone: 390812539449. Fax: 390812539407. E-mail: ercolini{at}unina.it.

{triangledown} Published ahead of print on 9 February 2007.

Applied and Environmental Microbiology, April 2007, p. 2354-2359, Vol. 73, No. 7
0099-2240/07/$08.00+0     doi:10.1128/AEM.02603-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ercolini, D., Russo, F., Nasi, A., Ferranti, P., Villani, F. (2009). Mesophilic and Psychrotrophic Bacteria from Meat and Their Spoilage Potential In Vitro and in Beef. Appl. Environ. Microbiol. 75: 1990-2001 [Abstract] [Full Text]  


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