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Applied and Environmental Microbiology, May 2007, p. 3091-3094, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02940-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Improved Luciferase Tagging System for Listeria monocytogenes Allows Real-Time Monitoring In Vivo and In Vitro{triangledown}

Christian U. Riedel,1 Ian R. Monk,1 Pat G. Casey,1 David Morrissey,2 Gerald C. O'Sullivan,2 Mark Tangney,2 Colin Hill,1* and Cormac G. M. Gahan1

Alimentary Pharmabiotic Centre and Department of Microbiology,1 Cork Cancer Research Centre, University College Cork, Cork, Ireland2

Received 19 December 2006/ Accepted 26 February 2007

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

* Corresponding author. Mailing address: Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353 (0)21 4901373. Fax: 353 (0)21 4903101. E-mail: c.hill{at}ucc.ie

{triangledown} Published ahead of print on 9 March 2007.

Applied and Environmental Microbiology, May 2007, p. 3091-3094, Vol. 73, No. 9
0099-2240/07/$08.00+0     doi:10.1128/AEM.02940-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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