This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomita, T.
Right arrow Articles by Deighton, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomita, T.
Right arrow Articles by Deighton, M.
Agricola
Right arrow Articles by Tomita, T.
Right arrow Articles by Deighton, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2008, p. 114-124, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01373-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Streptococcus uberis Multilocus Sequence Types Highly Associated with Mastitis{triangledown}

Takehiro Tomita,1 Brian Meehan,1 Nalin Wongkattiya,1 Jakob Malmo,2 Gillian Pullinger,3 James Leigh,4 and Margaret Deighton1*

Department of Biotechnology and Environmental Biology, RMIT University, Bundoora West Campus, Bundoora, Melbourne, Victoria 3083, Australia,1 University of Melbourne, Rural Veterinary Unit, Maffra, Victoria 3860, Australia,2 Institute for Animal Health, Compton Laboratory, Compton RG20 7NN, United Kingdom,3 School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom4

Received 21 June 2007/ Accepted 1 November 2007

Multilocus sequence typing analysis of Streptococcus uberis has identified a cluster of isolates associated with clinical and subclinical mastitis and a cluster associated with cows with low somatic cell counts in their milk. Specific groups of genotypes (global clonal complex [GCC] sequence type 5s [ST5s] and GCC ST143s) were highly associated (P = 0.006) with clinical and subclinical mastitis and may represent a lineage of virulent isolates, whereas isolates belonging to GCC ST86 were associated with low-cell-count cows. This study has, for the first time, demonstrated the occurrence of identical sequence types (ST60 and ST184) between different continents (Australasia and Europe) and different countries (Australia and New Zealand). The standardized index of association and the empirical estimation of the rate of recombination showed substantial recombination within the S. uberis population in Australia, consistent with previous multilocus sequence type analyses.

* Corresponding author. Mailing address: Department of Biotechnology and Environmental Biology, RMIT University, Bundoora West Campus, Bundoora, Melbourne, Victoria 3083, Australia. Phone: 61 3 9925 7123. Fax: 61 3 9925 7110. E-mail: m_deighton{at}rmit.edu.au

{triangledown} Published ahead of print on 16 November 2007.

Applied and Environmental Microbiology, January 2008, p. 114-124, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01373-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rato, M. G., Bexiga, R., Nunes, S. F., Cavaco, L. M., Vilela, C. L., Santos-Sanches, I. (2008). Molecular Epidemiology and Population Structure of Bovine Streptococcus uberis. J DAIRY SCI 91: 4542-4551 [Abstract] [Full Text]  
  • Pitkala, A., Koort, J., Bjorkroth, J. (2008). Identification and Antimicrobial Resistance of Streptococcus uberis and Streptococcus parauberis Isolated from Bovine Milk Samples. J DAIRY SCI 91: 4075-4081 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»