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Applied and Environmental Microbiology, January 2008, p. 336-341, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01522-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genomic Markers for Differentiation of Francisella tularensis subsp. tularensis A.I and A.II Strains{triangledown} ,{dagger}

Claudia R. Molins-Schneekloth,1,2 John T. Belisle,2 and Jeannine M. Petersen1*

Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado,1 Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado2

Received 28 June 2007/ Accepted 30 October 2007

Tularemia is caused by two subspecies of Francisella tularensis, F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holarctica (type B). F. tularensis subsp. tularensis is further subdivided into two genetically distinct populations (A.I and A.II) that differ with respect to geographical location, anatomical source of recovered isolates, and disease outcome. Using two human clinical isolates, suppression subtractive hybridization was performed to identify 13 genomic regions of difference between A.I and A.II strains. Two PCR assays, one to identify A.I and A.II as well as to discriminate between F. tularensis subsp. holarctica and F. novicida and another specific for A.I, were developed. This is the first report to identify and characterize conserved genomic differences between A.I and A.II.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Bacterial Diseases Branch, 3150 Rampart Road, Fort Collins, CO 80521. Phone: (970) 266-3524. Fax: (970) 494-6631. E-mail: JPetersen{at}cdc.gov

{triangledown} Published ahead of print on 16 November 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2008, p. 336-341, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.01522-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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