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Applied and Environmental Microbiology, January 2008, p. 99-106, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.00745-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of Two Lactobacillus casei Genes Encoding a Cystathionine Lyase{triangledown}

Stefan Irmler,1* Sylvie Raboud,1 Beata Beisert,2 Doris Rauhut,2 and Hélène Berthoud1

Agroscope Liebefeld-Posieux Research Station ALP, Bern, Switzerland,1 Geisenheim Research Center, Geisenheim, Germany2

Received 3 April 2007/ Accepted 29 October 2007

Volatile sulfur compounds are key flavor compounds in several cheese types. To better understand the metabolism of sulfur-containing amino acids, which certainly plays a key role in the release of volatile sulfur compounds, we searched the genome database of Lactobacillus casei ATCC 334 for genes encoding putative homologs of enzymes known to degrade cysteine, cystathionine, and methionine. The search revealed that L. casei possesses two genes that putatively encode a cystathionine β-lyase (CBL; EC 4.4.1.8). The enzyme has been implicated in the degradation of not only cystathionine but also cysteine and methionine. Recombinant CBL proteins catalyzed the degradation of L-cystathionine, O-succinyl-L-homoserine, L-cysteine, L-serine, and L-methionine to form {alpha}-keto acid, hydrogen sulfide, or methanethiol. The two enzymes showed notable differences in substrate specificity and pH optimum.

* Corresponding author. Mailing address: Agroscope Liebefeld-Posieux Research Station ALP, Schwarzenburgstrasse 161, Bern, Switzerland. Phone: 41 31 323 8155. Fax: 41-031 323 8227. E-mail: stefan.irmler{at}alp.admin.ch

{triangledown} Published ahead of print on 11 September 2007.

Applied and Environmental Microbiology, January 2008, p. 99-106, Vol. 74, No. 1
0099-2240/08/$08.00+0     doi:10.1128/AEM.00745-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lo, R., Turner, M. S., Barry, D. G., Sreekumar, R., Walsh, T. P., Giffard, P. M. (2009). Cystathionine {gamma}-Lyase Is a Component of Cystine-Mediated Oxidative Defense in Lactobacillus reuteri BR11. J. Bacteriol. 191: 1827-1837 [Abstract] [Full Text]  


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