This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bunescu, A.
Right arrow Articles by Delort, A.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bunescu, A.
Right arrow Articles by Delort, A.-M.
Agricola
Right arrow Articles by Bunescu, A.
Right arrow Articles by Delort, A.-M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2008, p. 2976-2984, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.01696-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Microbial and Photochemical Processes and Their Combination for Degradation of 2-Aminobenzothiazole{triangledown}

Andrei Bunescu,1,2,3 Pascale Besse-Hoggan,1 Martine Sancelme,1 Gilles Mailhot,2 and Anne-Marie Delort1*

Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 CNRS—Université Blaise Pascal, 63177 Aubière Cedex, France,1 Laboratoire de Photochimie Moléculaire et Macromoléculaire, UMR 6505 CNRS—Université Blaise Pascal, 63177 Aubière Cedex, France,2 Laboratory of Organic Chemistry, State University of Moldova, 60 A. Mateevici Str., Chisinau md-2009, Republic of Moldova3

Received 24 July 2007/ Accepted 20 February 2008

The transformation of 2-aminobenzothiazole (ABT) was studied under various conditions: (i) a photodegradation process at a {lambda} of >300 nm in the presence of an Fe(III)-nitrilotriacetic acid complex (FeNTA), (ii) a biodegradation process using Rhodococcus rhodochrous OBT18 cells, and (iii) the combined processes (FeNTA plus Rhodococcus rhodochrous in the presence or absence of light). The transformation of ABT in the combined system, with or without light, was much more efficient (99% degradation after 25 h) than in the separated systems (37% photodegradation and 26% biodegradation after 125 h). No direct photolysis of ABT was observed. The main result seen is the strong positive impact of FeNTA on the photodegradation, as expected, and on the biotransformation efficiency of ABT, which was more surprising. This positive impact of FeNTA on the microbial metabolism was dependent on the FeNTA concentration. The use of UV high-performance liquid chromatography, liquid chromatography-electrospray ionization mass spectrometry, and in situ 1H nuclear magnetic resonance provided evidence of the intermediary products and thus established transformation pathways of ABT in the different processes. These pathways were identical whether the degradation process was photo- or biotransformation. A new photoproduct was identified (4OH-ABT), corresponding to a hydroxylation reaction on position 4 of the aromatic ring of ABT.

* Corresponding author. Mailing address: Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 CNRS—Université Blaise Pascal, 63177 Aubière Cedex, France. Phone: 33 (0)4 73 40 77 14. Fax: 33 (0)4 73 40 77 17. E-mail: A-Marie.Delort{at}univ-bpclermont.fr

{triangledown} Published ahead of print on 29 February 2008.

Applied and Environmental Microbiology, May 2008, p. 2976-2984, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.01696-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bunescu, A., Besse-Hoggan, P., Sancelme, M., Mailhot, G., Delort, A.-M. (2008). Fate of the Nitrilotriacetic Acid-Fe(III) Complex during Photodegradation and Biodegradation by Rhodococcus rhodochrous. Appl. Environ. Microbiol. 74: 6320-6326 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»