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Applied and Environmental Microbiology, May 2008, p. 3048-3057, Vol. 74, No. 10
0099-2240/08/$08.00+0 doi:10.1128/AEM.02548-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Phylogenetic Analysis of Members of the Phycodnaviridae Virus Family, Using Amplified Fragments of the Major Capsid Protein Gene
J. B. Larsen,*
A. Larsen,
G. Bratbak, and
R.-A. Sandaa
Department of Biology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway
Received 12 November 2007/ Accepted 13 March 2008
Algal viruses are considered ecologically important by affecting host population dynamics and nutrient flow in aquatic food webs. Members of the family Phycodnaviridae are also interesting due to their extraordinary genome size. Few algal viruses in the Phycodnaviridae family have been sequenced, and those that have been have few genes in common and low gene homology. It has hence been difficult to design general PCR primers that allow further studies of their ecology and diversity. In this study, we screened the nine type I core genes of the nucleocytoplasmic large DNA viruses for sequences suitable for designing a general set of primers. Sequence comparison between members of the Phycodnaviridae family, including three partly sequenced viruses infecting the prymnesiophyte Pyramimonas orientalis and the haptophytes Phaeocystis pouchetii and Chrysochromulina ericina (Pyramimonas orientalis virus 01B [PoV-01B], Phaeocystis pouchetii virus 01 [PpV-01], and Chrysochromulina ericina virus 01B [CeV-01B], respectively), revealed eight conserved regions in the major capsid protein (MCP). Two of these regions also showed conservation at the nucleotide level, and this allowed us to design degenerate PCR primers. The primers produced 347- to 518-bp amplicons when applied to lysates from algal viruses kept in culture and from natural viral communities. The aim of this work was to use the MCP as a proxy to infer phylogenetic relationships and genetic diversity among members of the Phycodnaviridae family and to determine the occurrence and diversity of this gene in natural viral communities. The results support the current legitimate genera in the Phycodnaviridae based on alga host species. However, while placing the mimivirus in close proximity to the type species, PBCV-1, of Phycodnaviridae along with the three new viruses assigned to the family (PoV-01B, PpV-01, and CeV-01B), the results also indicate that the coccolithoviruses and phaeoviruses are more diverged from this group. Phylogenetic analysis of amplicons from virus assemblages from Norwegian coastal waters as well as from isolated algal viruses revealed a cluster of viruses infecting members of the prymnesiophyte and prasinophyte alga divisions. Other distinct clusters were also identified, containing amplicons from this study as well as sequences retrieved from the Sargasso Sea metagenome. This shows that closely related sequences of this family are present at geographically distant locations within the marine environment.
* Corresponding author. Mailing address: Institute of Biology, University of Bergen, Jahnebakken 5, N-5020 Bergen, Norway. Phone: 47 55584644. Fax: 47 55589671. E-mail: jens.larsen{at}bio.uib.no
Published ahead of print on 21 March 2008.
Applied and Environmental Microbiology, May 2008, p. 3048-3057, Vol. 74, No. 10
0099-2240/08/$08.00+0 doi:10.1128/AEM.02548-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.
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