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Applied and Environmental Microbiology, May 2008, p. 3266-3268, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.00059-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Induction and Resuscitation of Viable Nonculturable Arcobacter butzleri Cells{triangledown}

M. T. Fera,1* T. L. Maugeri,2 C. Gugliandolo,2 E. La Camera,1 V. Lentini,2 A. Favaloro,3 D. Bonanno,4 and M. Carbone1

Dipartimento di Patologia e Microbiologia Sperimentale,1 Dipartimento di Biologia Animale ed Ecologia Marina,2 Dipartimento di Biomorfologia e Biotecnologie,3 Dipartimento di Patologia Umana, Università di Messina, 98100 Messina, Italy4

Received 8 January 2008/ Accepted 18 March 2008

Two strains of Arcobacter butzleri, ATCC 49616 and an environmental isolate, became nonculturable in seawater microcosms at 4°C by 20 days and at room temperature by 14 days. Nonculturable cells were viable for up to 270 days of incubation in microcosms. Resuscitation of A. butzleri cells from microcosms at both temperatures was achieved 9 days after nutrient addition.

* Corresponding author. Mailing address: Dipartimento di Patologia e Microbiologia Sperimentale, Facoltà di Medicina e Chirurgia, Policlinico Universitario, Torre Biologica, 2° piano, Università di Messina, 98125 Messina, Italy. Phone: 39-090-2213313. Fax: 39-090-2213312. E-mail: mariateresa.fera{at}unime.it

{triangledown} Published ahead of print on 31 March 2008.

Applied and Environmental Microbiology, May 2008, p. 3266-3268, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.00059-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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