This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liles, M. R.
Right arrow Articles by Handelsman, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liles, M. R.
Right arrow Articles by Handelsman, J.
Agricola
Right arrow Articles by Liles, M. R.
Right arrow Articles by Handelsman, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2008, p. 3302-3305, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02630-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Recovery, Purification, and Cloning of High-Molecular-Weight DNA from Soil Microorganisms{triangledown}

Mark R. Liles,1 Lynn L. Williamson,2 Jitsupang Rodbumrer,3 Vigdis Torsvik,4 Robert M. Goodman,5* and Jo Handelsman2

Department of Biological Sciences, Auburn University, Auburn, Alabama,1 Department of Bacteriology, University of Wisconsin, Madison, Wisconsin,2 Department of Biotechnology, Mahidol University, Bangkok, Thailand,3 Department of Microbiology, University of Bergen, Bergen, Norway,4 School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey5

Received 20 November 2007/ Accepted 12 March 2008

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.

* Corresponding author. Mailing address: Rutgers, The State University of New Jersey, School of Environmental and Biological Sciences, 88 Lipman Drive, Suite 104, New Brunswick, NJ 08901. Phone: (732) 932-9000, ext. 501. Fax: (732) 932-8955. E-mail: rgoodman{at}aesop.rutgers.edu

{triangledown} Published ahead of print on 21 March 2008.

Applied and Environmental Microbiology, May 2008, p. 3302-3305, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02630-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liles, M. R., Williamson, L. L., Rodbumrer, J., Torsvik, V., Parsley, L. C., Goodman, R. M., Handelsman, J. (2009). Isolation and Cloning of High-Molecular-Weight Metagenomic DNA from Soil Microorganisms. CSH Protocols 2009: pdb.prot5271-pdb.prot5271 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»