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Applied and Environmental Microbiology, May 2008, p. 3319-3320, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02623-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Transcription-Mediated Amplification and Growth-Based Methods for the Quantitation of Enterococcus Bacteria in Environmental Waters{triangledown}

Ceri A. Morris,1* A. Denene Blackwood,2 Marek Kirs,2 Neil D. Buttigieg,1 Rhian R. Morgan,1 James J. Hogan,3 Ian Weeks,1 and Rachel T. Noble2

MLT Research Ltd., 5 Chiltern Close, Cardiff CF14 5DL, United Kingdom,1 University of North Carolina at Chapel Hill, Institute of Marine Sciences, Morehead City, North Carolina 28557,2 Gen-Probe Incorporated, 10210 Genetic Center Drive, San Diego, California 921213

Received 20 November 2007/ Accepted 23 March 2008

An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.

* Corresponding author. Mailing address: Research and Development, MLT Research Ltd., 5 Chiltern Close, Cardiff Industrial Park, Cardiff CF14 5DL, United Kingdom. Phone: 44 (0)29 20747033. Fax: 44 (0)29 20747118. E-mail: ceri.morris{at}mltresearch.com

{triangledown} Published ahead of print on 31 March 2008.

Applied and Environmental Microbiology, May 2008, p. 3319-3320, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02623-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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