This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental materials
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanaka, M.
Right arrow Articles by Matsunaga, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, M.
Right arrow Articles by Matsunaga, T.
Agricola
Right arrow Articles by Tanaka, M.
Right arrow Articles by Matsunaga, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2008, p. 3342-3348, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02276-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of a Cell Surface Display System in a Magnetotactic Bacterium, "Magnetospirillum magneticum" AMB-1 {triangledown} ,{dagger}

Masayoshi Tanaka,1 Yuko Nakata,1 Tetsushi Mori,1 Yoshiko Okamura,1 Hitoshi Miyasaka,2 Haruko Takeyama,1 and Tadashi Matsunaga1*

Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan,1 The Kansai Electric Power Co., Environmental Research Center, Keihanna-Plaza 12F, Hikaridai 1-7 Seikacho, Sourakugun, Kyoto 619-0237, Japan2

Received 6 October 2007/ Accepted 24 March 2008

Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work, using this unique characteristic and based on whole-genomic and comprehensive proteomic analyses of these bacteria, a cell surface display system has been developed by expressing hexahistidine residues within the outer coiled loop of the membrane-specific protein (Msp1) of the "Magnetospirillum magneticum" (proposed name) AMB-1 bacterium. The optimal display site of the hexahistidine residues was successfully identified via secondary structure prediction, immunofluorescence microscopy, and heavy metal binding assay. The established AMB-1 transformant showed high immunofluorescence response, high Cd2+ binding, and high recovery efficiency in comparison to those of the negative control when manipulated by magnetic force.

* Corresponding author. Mailing address: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. Phone: 81-42-388-7022. Fax: 81-42-385-7713. E-mail: tmatsuna{at}cc.tuat.ac.jp

{triangledown} Published ahead of print on 31 March 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2008, p. 3342-3348, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02276-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»