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Applied and Environmental Microbiology, June 2008, p. 3915-3917, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02807-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transfer of Conjugative Elements from Rumen and Human Firmicutes Bacteria to Roseburia inulinivorans{triangledown}

Karen P. Scott,* Jenny C. Martin, Jakub Mrazek,{dagger} and Harry J. Flint

Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom

Received 12 December 2007/ Accepted 22 April 2008

Studies on Firmicutes bacteria from the gut are hampered by a lack of gene transfer systems. Here the human colonic anaerobe Roseburia inulinivorans A2-194 was shown to be a transfer recipient for two conjugative transposons, Tn1545 from Eubacterium cellulosolvens and TnK10 from Clostridium saccharolyticum K10.

* Corresponding author. Mailing address: Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, Scotland AB21 9SB, United Kingdom. Phone: 44 (0) 1224 712751. Fax: 44 (0) 1224 716687. E-mail: k.scott{at}rowett.ac.uk

{triangledown} Published ahead of print on 2 May 2008.

{dagger} Present address: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic.

Applied and Environmental Microbiology, June 2008, p. 3915-3917, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02807-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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