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Applied and Environmental Microbiology, July 2008, p. 4314-4323, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02821-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genotypic Characterization and Prevalence of Virulence Factors among Canadian Escherichia coli O157:H7 Strains{triangledown} ,{dagger}

Kim Ziebell,1 Marina Steele,1 Yongxiang Zhang,2 Andrew Benson,3 Eduardo N. Taboada,2 Chad Laing,2 Scott McEwen,4 Bruce Ciebin,5 Roger Johnson,1 and Victor Gannon2*

Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada,1 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta T1J 3Z4, Canada,2 Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska 68583-0919,3 Department of Population Medicine, University of Guelph, Guelph, Ontario N1G 2W1, Canada,4 Ontario Ministry of Health and Long-Term Care, Hamilton Public Health Laboratory, Hamilton, Ontario L8N 3R5, Canada5

Received 13 December 2007/ Accepted 1 May 2008

In this study, the association between genotypic and selected phenotypic characteristics was examined in a collection of Canadian Escherichia coli O157:H7 strains isolated from humans and cattle in the provinces of Alberta, Ontario, Saskatchewan, and Quebec. In a subset of 69 strains selected on the basis of specific phage types (PTs), a strong correlation between the lineage-specific polymorphism assay (LSPA6) genotype and PT was observed with all strains of PTs 4, 14, 21, 31, 33, and 87 belonging to the LSPA6 lineage I (LSPA6-LI) genotype, while those of PTs 23, 45, 67, and 74 belonged to LSPA6 lineage II (LSPA6-LII) genotypes. This correlation was maintained when additional strains of each PT were tested. E. coli O157:H7 strains with the LSPA6-LI genotype were much more common in the collection than were the LSPA6-LII or lineage I/II (LSPA6-LI/II)-related genotypes (82.6, 11.2, and 5.8%, respectively). Of the strains tested, proportionately more LSPA6-LI than LSPA6-LII genotype strains were isolated from humans (52.7% versus 19.7%) than from cattle (47.8% versus 80.2%). In addition, 96.7% of the LSPA6-LII strains carried the stx2c variant gene, while only 50.0% of LSPA6-LI/II and 2.7% of LSPA6-LI strains carried this gene. LSPA6-LII strains were also significantly more likely to possess the colicin D gene, cda (50.8% versus 23.2%), and have combined resistance to streptomycin, sulfisoxazole, and tetracycline (72.1% versus 0.9%) than were LSPA6-LI strains. The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics.

* Corresponding author. Mailing address: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, C/O CFIA Lethbridge Laboratory, Box 640, Townshiproad 9-1, Lethbridge, Alberta T1J 3Z4, Canada. Phone: (403) 382-5514. Fax: (403) 381-1202. E-mail: gannonv{at}inspection.gc.ca

{triangledown} Published ahead of print on 16 May 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2008, p. 4314-4323, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02821-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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