This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bullen, H. A.
Right arrow Articles by Barton, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bullen, H. A.
Right arrow Articles by Barton, H. A.
Agricola
Right arrow Articles by Bullen, H. A.
Right arrow Articles by Barton, H. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2008, p. 4553-4559, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02936-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy To Identify Microbial Metabolic Products on Carbonate Mineral Surfaces{triangledown} ,{dagger}

Heather A. Bullen,1 Stuart A. Oehrle,1 Ariel F. Bennett,2 Nicholas M. Taylor,2 and Hazel A. Barton2*

Departments of Chemistry,1 Biological Sciences, Northern Kentucky University, Highland Heights, Kentucky 410992

Received 30 December 2007/ Accepted 13 May 2008

This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to distinguish metabolic acids on calcite surfaces following Escherichia coli growth. The production of these acids by E. coli was verified using high-performance liquid chromatography with refractive index detection. The development of this technique has allowed us to identify microbial metabolic products on carbonate surfaces in nutrient-limited cave environments.

* Corresponding author. Mailing address: Department of Biological Sciences, Northern Kentucky University, SC 204D Nunn Drive, Highland Heights, KY 41099. Phone: (859) 572-5303. Fax: (859) 572-5639. E-mail: bartonh{at}nku.edu

{triangledown} Published ahead of print on 23 May 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2008, p. 4553-4559, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02936-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»