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Applied and Environmental Microbiology, August 2008, p. 5220-5223, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00369-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Bacillus anthracis Spores by Multiparameter Flow Cytometry {triangledown}

William C. Schumacher,1 Craig A. Storozuk,2,3 Prabir K. Dutta,1 and Andrew J. Phipps2,3*

Department of Chemistry,1 Department of Veterinary Biosciences,2 Center for Microbial Interface Biology, Ohio State University, Columbus, Ohio 432103

Received 13 February 2008/ Accepted 20 June 2008

In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifying B. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.

* Corresponding author. Present address: Battelle, 505 King Avenue, Columbus, OH 43201. Phone: (410) 306-8526. Fax: (614) 458-6727. E-mail: PhippsA{at}battelle.org

{triangledown} Published ahead of print on 27 June 2008.

Applied and Environmental Microbiology, August 2008, p. 5220-5223, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00369-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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