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Applied and Environmental Microbiology, August 2008, p. 5224-5227, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00621-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species{triangledown}

Tanja Riess,1,{dagger} Florian Dietrich,1,{dagger} Katja V. Schmidt,1 Patrick O. Kaiser,1 Heinz Schwarz,2 Andrea Schäfer,1 and Volkhard A. J. Kempf1*

Institut für Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universität, Elfriede-Aulhorn-Str. 6, 72076 Tübingen, Germany,1 Max-Planck-Institut für Entwicklungsbiologie, Elektronenmikroskopie, Spemannstr. 35, 72076 Tübingen, Germany2

Received 14 March 2008/ Accepted 10 June 2008

Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host cells.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Elfriede-Aulhorn-Str. 6, D-72076 Tübingen, Germany. Phone: 49-7071-2982352. Fax: 49-7071-295440. E-mail: volkhard.kempf{at}med.uni-tuebingen.de

{triangledown} Published ahead of print on 20 June 2008.

{dagger} T.R. and F.D. contributed equally to this work.

Applied and Environmental Microbiology, August 2008, p. 5224-5227, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00621-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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