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Applied and Environmental Microbiology, August 2008, p. 5250-5253, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00272-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy-L-Sorbose{triangledown}

Xue-Peng Yang,1,2,{dagger} Liu-Jing Wei,1,{dagger} Jin-Ping Lin,1 Bo Yin,1 and Dong-Zhi Wei1*

State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China,1 College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China2

Received 31 January 2008/ Accepted 12 May 2008

A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.

* Corresponding author. Mailing address: East China University of Science and Biotechnology, Mei-Long 130, Shanghai 200237, China. Phone: 86-21-64252981. Fax: 86-21-64250068. E-mail: dzhwei{at}ecust.edu.cn

{triangledown} Published ahead of print on 23 May 2008.

{dagger} These authors contributed equally to this work.

Applied and Environmental Microbiology, August 2008, p. 5250-5253, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00272-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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