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Applied and Environmental Microbiology, September 2008, p. 5290-5296, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00832-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of a Gene Encoding a Transporter Essential for Utilization of C4 Dicarboxylates in Corynebacterium glutamicum{triangledown}

Haruhiko Teramoto, Tomokazu Shirai, Masayuki Inui, and Hideaki Yukawa*

Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan

Received 11 April 2008/ Accepted 19 June 2008

The Corynebacterium glutamicum R genome contains a total of eight genes encoding proteins with sequence similarity to C4-dicarboxylate transporters identified from other bacteria. Three of the genes encode proteins within the dicarboxylate/amino acid:cation symporter (DAACS) family, another three encode proteins within the tripartite ATP-independent periplasmic transporter family, and two encode proteins within the divalent anion:Na+ symporter (DASS) family. We observed that a mutant strain deficient in one of these genes, designated dcsT, of the DASS family did not aerobically grow on the C4 dicarboxylates succinate, fumarate, and malate as the sole carbon sources. Mutant strains deficient in each of the other seven genes grew as well as the wild-type strain under the same conditions, although one of these genes is a homologue of dctA of the DAACS family, involved in aerobic growth on C4 dicarboxylates in various bacteria. The utilization of C4 dicarboxylates was markedly enhanced by overexpression of the dcsT gene. We confirmed that the uptake of [13C]labeled succinate observed for the wild-type cells was hardly detected in the dcsT-deficient mutant but was markedly enhanced in a dcsT-overexpressing strain. These results suggested that in C. glutamicum, the uptake of C4 dicarboxylates for aerobic growth was mainly mediated by the DASS transporter encoded by dcsT. The expression level of the dcsT gene transiently increased in the early exponential phase during growth on nutrient-rich medium. This expression was enhanced by the addition of succinate in the mid-exponential phase and was repressed by the addition of glucose in the early exponential phase.

* Corresponding author. Mailing address: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan. Phone: 81-774-75-2308. Fax: 81-774-75-2321. E-mail: mmg-lab{at}rite.or.jp

{triangledown} Published ahead of print on 27 June 2008.

Applied and Environmental Microbiology, September 2008, p. 5290-5296, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00832-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Youn, J.-W., Jolkver, E., Kramer, R., Marin, K., Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. J. Bacteriol. 191: 5480-5488 [Abstract] [Full Text]  
  • Teramoto, H., Inui, M., Yukawa, H. (2009). Regulation of Expression of Genes Involved in Quinate and Shikimate Utilization in Corynebacterium glutamicum. Appl. Environ. Microbiol. 75: 3461-3468 [Abstract] [Full Text]  
  • Jolkver, E., Emer, D., Ballan, S., Kramer, R., Eikmanns, B. J., Marin, K. (2009). Identification and Characterization of a Bacterial Transport System for the Uptake of Pyruvate, Propionate, and Acetate in Corynebacterium glutamicum. J. Bacteriol. 191: 940-948 [Abstract] [Full Text]  


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