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Applied and Environmental Microbiology, September 2008, p. 5841-5844, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.01099-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

An Amino Acid Substitution at Position 740 in {sigma}70 of Ralstonia solanacearum Strain OE1-1 Affects Its In Planta Growth{triangledown}

Ayami Kanda,1,{dagger} Kazuhiro Tsuneishi,1 Ai Mori,1 Kouhei Ohnishi,2 Akinori Kiba,1 and Yasufumi Hikichi1*

Laboratory of Plant Pathology & Biotechnology, Kochi University, Nankoku, Kochi 783-8502, Japan,1 Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi 783-8502, Japan2

Received 15 May 2008/ Accepted 11 July 2008

Growth of Ralstonia solanacearum strain OE1-1 in roots after invasion is required for virulence. An Arg740Cys substitution in {sigma}70 of OE1-1 resulted in loss of in planta growth and virulence. The negative dominance of mutant {sigma}70 over the wild-type protein suggested that the amino acid substitution may affect the in planta growth of OE1-1, leading to a lack of virulence.

* Corresponding author. Mailing address: Faculty of Agriculture, Kochi University, 200 Monobe, Nankoku, Kochi 783-8502, Japan. Phone: 81-88-864-5218. Fax: 81-88-864-5200. E-mail: yhikichi{at}cc.kochi-u.ac.jp

{triangledown} Published ahead of print on 18 July 2008.

{dagger} Present address. National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.

Applied and Environmental Microbiology, September 2008, p. 5841-5844, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.01099-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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