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Applied and Environmental Microbiology, September 2008, p. 5850-5853, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00399-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quantification of Desulfovibrio vulgaris Dissimilatory Sulfite Reductase Gene Expression during Electron Donor- and Electron Acceptor-Limited Growth{triangledown}

Laura Villanueva,* Shelley A. Haveman, Zara M. Summers, and Derek R. Lovley

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts

Received 17 February 2008/ Accepted 15 July 2008

Previous studies have suggested that levels of transcripts for dsrA, a gene encoding a subunit of the dissimilatory sulfite reductase, are not directly related to the rates of sulfate reduction in sediments under all conditions. This phenomenon was further investigated with chemostat-grown Desulfovibrio vulgaris. Under sulfate-limiting conditions, dsrA transcript levels increased as the bulk rates of sulfate reduction in the chemostat increased, but transcript levels were similar at all sulfate reduction rates under electron donor-limiting conditions. When both electron donor- and electron acceptor-limiting conditions were considered, there was a direct correspondence between dsrA transcript levels and the rates of sulfate reduction per cell. These results suggest that dsrA transcript levels may provide important information on the metabolic state of sulfate reducers.

* Corresponding author. Present address: Harvard FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138. Phone: (617) 496-9519. Fax: (617) 495-2196. E-mail: lvillanueva{at}cgr.harvard.edu

{triangledown} Published ahead of print on 25 July 2008.

Applied and Environmental Microbiology, September 2008, p. 5850-5853, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00399-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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