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Applied and Environmental Microbiology, September 2008, p. 5854-5856, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.01291-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Enhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System{triangledown}

D. M. Villarreal,1 C. L. Phillips,1 A. M. Kelley,1 S. Villarreal,1 A. Villaloboz,1 P. Hernandez,1 J. S. Olson,2 and D. P. Henderson1*

Department of Biology, University of Texas of the Permian Basin, Odessa, Texas 79762,1 Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 770052

Received 10 June 2008/ Accepted 20 July 2008

To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.

* Corresponding author. Mailing address: Department of Biology, University of Texas of the Permian Basin, 4901 East University Blvd., Odessa, TX 79762-0001. Phone: (432) 552-2270. Fax: (432) 552-3230. E-mail: henderson_d{at}utpb.edu

{triangledown} Published ahead of print on 1 August 2008.

Applied and Environmental Microbiology, September 2008, p. 5854-5856, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.01291-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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