This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Appunu, C. Articles by Laguerre, G. Search for Related Content PubMed PubMed Citation Articles by Appunu, C. Articles by Laguerre, G. Agricola Articles by Appunu, C. Articles by Laguerre, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2008, p. 5991-5996, Vol. 74, No. 19
0099-2240/08/$08.00+0     doi:10.1128/AEM.01320-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans (Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India

Chinnaswamy Appunu,^1,2,3, Angèle N'Zoue,^4,5,6,7,8 and Gisèle Laguerre^{2,3,4,5,6,7,8*}

Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India,¹ INRA, UMR 1229 Microbiologie et Géochimie des Sols, F-21065 Dijon, France,² Université de Bourgogne, UMR 1229, F-21065 Dijon, France,³ IRD, UMR 113 Symbioses Tropicales et Méditerranéennes, F-34398 Montpellier, France,⁴ INRA, UMR 113, F-34398 Montpellier, France,⁵ CIRAD, UMR 113, F-34398 Montpellier, France,⁶ SupAgro, UMR 113, F-34398 Montpellier, France,⁷ Université de Montpellier II, UMR 113, F-34398 Montpellier, France⁸

Received 13 June 2008/ Accepted 28 July 2008

Fifty isolates from root nodules of soybean plants sampled in five agricultural-ecological-climatic regions of India were analyzed by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene, the intergenic spacer region between the 16S and 23S rRNA genes (IGS), and the nifH and nodC genes. Eight haplotypes assigned to the Bradyrhizobium genus were identified, and the genetic diversity was conserved across regions. Sequence analyses of the IGS and the dnaK, glnII, recA, and nifH genes revealed three groups. One of them (26% of isolates) was assigned to Bradyrhizobium liaoningense. A second group (36% of isolates) was identified as B. yuanmingense but likely forms a new biovar able to nodulate soybean plants. The third lineage (38% of isolates) was different from all described Bradyrhizobium species but showed the same symbiotic genotype as B. liaoningense and B. japonicum bv. glycinearum.

---

* Corresponding author. Mailing address: USC INRA 1242-UMR 113 IRD-CIRAD-SupAgro-UM2, Laboratoire des Symbioses Tropicales et Méditerranéennes, TA A-82/J Campus de Baillarguet, F-34398 Montpellier Cedex 5, France. Phone: 33 4 67 59 38 62. Fax: 33 4 67 59 38 02. E-mail: gisele.laguerre{at}supagro.inra.fr

Published ahead of print on 1 August 2008.

Present address: Division of Microbiology, M. S. Swaminathan Research Foundation, Taramani Institutional Area, Third Cross Road, Chennai 620113, India.

---

Applied and Environmental Microbiology, October 2008, p. 5991-5996, Vol. 74, No. 19
0099-2240/08/$08.00+0     doi:10.1128/AEM.01320-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Vinuesa, P., Rojas-Jimenez, K., Contreras-Moreira, B., Mahna, S. K., Prasad, B. N., Moe, H., Selvaraju, S. B., Thierfelder, H., Werner, D. (2008). Multilocus Sequence Analysis for Assessment of the Biogeography and Evolutionary Genetics of Four Bradyrhizobium Species That Nodulate Soybeans on the Asiatic Continent. Appl. Environ. Microbiol. 74: 6987-6996 [Abstract] [Full Text]