This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Izano, E. A.
Right arrow Articles by Kaplan, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Izano, E. A.
Right arrow Articles by Kaplan, J. B.
Agricola
Right arrow Articles by Izano, E. A.
Right arrow Articles by Kaplan, J. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2008, p. 470-476, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.02073-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Differential Roles of Poly-N-Acetylglucosamine Surface Polysaccharide and Extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis Biofilms{triangledown}

Era A. Izano, Matthew A. Amarante, William B. Kher, and Jeffrey B. Kaplan*

Department of Oral Biology, New Jersey Dental School, Newark, New Jersey

Received 11 September 2007/ Accepted 13 November 2007

Staphylococcus aureus and Staphylococcus epidermidis are major human pathogens of increasing importance due to the dissemination of antibiotic-resistant strains. Evidence suggests that the ability to form matrix-encased biofilms contributes to the pathogenesis of S. aureus and S. epidermidis. In this study, we investigated the functions of two staphylococcal biofilm matrix polymers: poly-N-acetylglucosamine surface polysaccharide (PNAG) and extracellular DNA (ecDNA). We measured the ability of a PNAG-degrading enzyme (dispersin B) and DNase I to inhibit biofilm formation, detach preformed biofilms, and sensitize biofilms to killing by the cationic detergent cetylpyridinium chloride (CPC) in a 96-well microtiter plate assay. When added to growth medium, both dispersin B and DNase I inhibited biofilm formation by both S. aureus and S. epidermidis. Dispersin B detached preformed S. epidermidis biofilms but not S. aureus biofilms, whereas DNase I detached S. aureus biofilms but not S. epidermidis biofilms. Similarly, dispersin B sensitized S. epidermidis biofilms to CPC killing, whereas DNase I sensitized S. aureus biofilms to CPC killing. We concluded that PNAG and ecDNA play fundamentally different structural roles in S. aureus and S. epidermidis biofilms.

* Corresponding author. Mailing address: Medical Science Building, Room C-636, 185 S. Orange Ave., Newark, NJ 07103. Phone: (973) 972-9508. Fax: (973) 972-0045. E-mail: kaplanjb{at}umdnj.edu

{triangledown} Published ahead of print on 26 November 2007.

Applied and Environmental Microbiology, January 2008, p. 470-476, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.02073-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Opperman, T. J., Kwasny, S. M., Williams, J. D., Khan, A. R., Peet, N. P., Moir, D. T., Bowlin, T. L. (2009). Aryl Rhodanines Specifically Inhibit Staphylococcal and Enterococcal Biofilm Formation. Antimicrob. Agents Chemother. 53: 4357-4367 [Abstract] [Full Text]  
  • Wen, F., White, G. J., VanEtten, H. D., Xiong, Z., Hawes, M. C. (2009). Extracellular DNA Is Required for Root Tip Resistance to Fungal Infection. Plant Physiol. 151: 820-829 [Abstract] [Full Text]  
  • Amarasinghe, J. J., Scannapieco, F. A., Haase, E. M. (2009). Transcriptional and Translational Analysis of Biofilm Determinants of Aggregatibacter actinomycetemcomitans in Response to Environmental Perturbation. Infect. Immun. 77: 2896-2907 [Abstract] [Full Text]  
  • Stevens, N. T., Greene, C. M., O'Gara, J. P., Humphreys, H. (2009). Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis. J Med Microbiol 58: 855-862 [Abstract] [Full Text]  
  • Karatan, E., Watnick, P. (2009). Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms. Microbiol. Mol. Biol. Rev. 73: 310-347 [Abstract] [Full Text]  
  • Nostro, A., Marino, A., Blanco, A. R., Cellini, L., Di Giulio, M., Pizzimenti, F., Roccaro, A. S., Bisignano, G. (2009). In vitro activity of carvacrol against staphylococcal preformed biofilm by liquid and vapour contact. J Med Microbiol 58: 791-797 [Abstract] [Full Text]  
  • Vilain, S., Pretorius, J. M., Theron, J., Brozel, V. S. (2009). DNA as an Adhesin: Bacillus cereus Requires Extracellular DNA To Form Biofilms. Appl. Environ. Microbiol. 75: 2861-2868 [Abstract] [Full Text]  
  • Tetz, G. V., Artemenko, N. K., Tetz, V. V. (2009). Effect of DNase and Antibiotics on Biofilm Characteristics. Antimicrob. Agents Chemother. 53: 1204-1209 [Abstract] [Full Text]  
  • Luong, T. T., Lei, M. G., Lee, C. Y. (2009). Staphylococcus aureus Rbf Activates Biofilm Formation In Vitro and Promotes Virulence in a Murine Foreign Body Infection Model. Infect. Immun. 77: 335-340 [Abstract] [Full Text]  
  • Trotonda, M. P., Tamber, S., Memmi, G., Cheung, A. L. (2008). MgrA Represses Biofilm Formation in Staphylococcus aureus. Infect. Immun. 76: 5645-5654 [Abstract] [Full Text]  
  • Seidl, K., Goerke, C., Wolz, C., Mack, D., Berger-Bachi, B., Bischoff, M. (2008). Staphylococcus aureus CcpA Affects Biofilm Formation. Infect. Immun. 76: 2044-2050 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»