This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baschien, C.
Right arrow Articles by Szewzyk, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baschien, C.
Right arrow Articles by Szewzyk, U.
Agricola
Right arrow Articles by Baschien, C.
Right arrow Articles by Szewzyk, U.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2008, p. 6427-6436, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00815-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Situ Detection of Freshwater Fungi in an Alpine Stream by New Taxon-Specific Fluorescence In Situ Hybridization Probes{triangledown}

Christiane Baschien,1* Werner Manz,2 Thomas R. Neu,3 Ludmila Marvanová,4 and Ulrich Szewzyk1

Department of Environmental Microbiology, Berlin Institute of Technology, Franklinstrasse 29, 10587 Berlin, Germany,1 Department of Biochemistry and Ecotoxicology, Federal Institute of Hydrology, P.O. Box 20 02 53, 56002 Koblenz, Germany,2 Department of River Ecology, Helmholtz Centre for Environmental Research UFZ Leipzig-Halle, Brueckstrasse 3A, 39114 Magdeburg, Germany,3 Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdeho 14, 602 00 Brno, Czech Republic4

Received 9 April 2008/ Accepted 24 August 2008

New rRNA-targeting oligonucleotide probes permitted the fluorescence in situ hybridization (FISH) identification of freshwater fungi in an Austrian second-order alpine stream. Based on computer-assisted comparative sequence analysis, nine taxon-specific probes were designed and evaluated by whole-fungus hybridizations. Oligonucleotide probe MY1574, specific for a wide range of Eumycota, and the genus (Tetracladium)-specific probe TCLAD1395, as well as the species-specific probes ALacumi1698 (Alatospora acuminata), TRIang322 (Tricladium angulatum), and Alongi340 (Anguillospora longissima), are targeted against 18S rRNA, whereas probes TmarchB10, TmarchC1_1, TmarchC1_2, and AlongiB16 are targeted against the 28S rRNA of Tetracladium marchalianum and Anguillospora longissima, respectively. After 2 weeks and 3 months of exposure of polyethylene slides in the stream, attached germinating conidia and growing hyphae of freshwater fungi were accessible for FISH. Growing hyphae and germinating conidia on leaves and in membrane cages were also visualized by the new FISH probes.

* Corresponding author. Mailing address: Department of Environmental Microbiology, Berlin Institute of Technology, Franklinstrasse 29, 10587 Berlin, Germany. Phone: 49-30-31473677. Fax: 49-3031473673. E-mail: christiane.baschien{at}tu-berlin.de

{triangledown} Published ahead of print on 5 September 2008.

Applied and Environmental Microbiology, October 2008, p. 6427-6436, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00815-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»