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Applied and Environmental Microbiology, November 2008, p. 6811-6813, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluating the Insecticidal Genes and Their Expressed Products in Bacillus thuringiensis Strains by Combining PCR with Mass Spectrometry{triangledown} ,{dagger}

Yunjun Sun,{ddagger} Zujiao Fu,{ddagger} Xuezhi Ding, and Liqiu Xia*

College of Life Science, Hunan Normal University, Changsha 410081, People's Republic of China

Received 15 May 2008/ Accepted 3 September 2008

By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluating B. thuringiensis strains at both the gene and protein levels.

* Corresponding author. Mailing address: College of Life Science, Hunan Normal University, Changsha 410081, China. Phone: 86-731-8872298. Fax: 86-731-8872260. E-mail: xialq{at}hunnu.edu.cn

{triangledown} Published ahead of print on 12 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} These authors contributed equally to this work.

Applied and Environmental Microbiology, November 2008, p. 6811-6813, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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