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Applied and Environmental Microbiology, November 2008, p. 7051-7055, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01511-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Brucella abortus Strain RB51 Leucine Auxotroph as an Environmentally Safe Vaccine for Plasmid Maintenance and Antigen Overexpression{triangledown} ,{dagger}

Parthiban Rajasekaran,1 Mohamed N. Seleem,2 Andrea Contreras,1 Endang Purwantini,3 Gerhardt G. Schurig,1 Nammalwar Sriranganathan,1 and Stephen M. Boyle1*

Department of Biomedical Sciences and Pathobiology and Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine,1 Institute for Critical Technology and Applied Science,2 Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 240613

Received 3 July 2008/ Accepted 17 September 2008

To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.

* Corresponding author. Mailing address: 1410 Price's Fork Road, CMMID, Blacksburg, VA 24061-0342. Phone: (540) 231-4641. Fax: (540) 231-3426. E-mail: smboyle{at}vt.edu

{triangledown} Published ahead of print on 3 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2008, p. 7051-7055, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01511-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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