This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lyell, N. L.
Right arrow Articles by Stabb, E. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lyell, N. L.
Right arrow Articles by Stabb, E. V.
Agricola
Right arrow Articles by Lyell, N. L.
Right arrow Articles by Stabb, E. V.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2008, p. 7059-7063, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01330-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effective Mutagenesis of Vibrio fischeri by Using Hyperactive Mini-Tn5 Derivatives{triangledown}

Noreen L. Lyell,1 Anne K. Dunn,1,2 Jeffrey L. Bose,1 Susan L. Vescovi,1 and Eric V. Stabb1*

Department of Microbiology, University of Georgia, Athens, Georgia 30602,1 Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma2

Received 13 June 2008/ Accepted 16 September 2008

We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of ~10–6. This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of V. fischeri and should be applicable to many other bacteria.

* Corresponding author. Mailing address: University of Georgia, Department of Microbiology, 1000 Cedar Street, Athens, GA 30602. Phone: (706) 542-2414. Fax: (706) 542-2674. E-mail: estabb{at}uga.edu

{triangledown} Published ahead of print on 19 September 2008.

Applied and Environmental Microbiology, November 2008, p. 7059-7063, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01330-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»