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Applied and Environmental Microbiology, November 2008, p. 7085-7089, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01491-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of {alpha}-Methylacyl Coenzyme A Racemase from Gordonia polyisoprenivorans VH2{triangledown} ,{dagger}

Quyen Arenskötter, Jens Heller, David Dietz, Matthias Arenskötter, and Alexander Steinbüchel*

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany

Received 2 July 2008/ Accepted 18 September 2008

The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcrVH2 expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes coding for a putative cytochrome P450 monooxygenase and a short-chain dehydrogenase/reductase involved in β-oxidation and poly(cis-1,4-isoprene) degradation were also characterized.

* Corresponding author. Mailing address: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Correnstrasse 3, D-48149 Münster, Germany. Phone: 49-251-8339821. Fax: 49-251-8338388. E-mail: steinbu{at}uni-muenster.de

{triangledown} Published ahead of print on 26 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2008, p. 7085-7089, Vol. 74, No. 22
0099-2240/08/$08.00+0     doi:10.1128/AEM.01491-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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