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Applied and Environmental Microbiology, December 2008, p. 7442-7446, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.00638-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

HtrA Is Essential for Efficient Secretion of Recombinant Proteins by Lactococcus lactis{triangledown}

Kalpana Sriraman and Guhan Jayaraman*

Department of Biotechnology, Indian Institute of Technology—Madras, Chennai, India 600036

Received 15 March 2008/ Accepted 23 September 2008

HtrA is a unique protease on the extracellular surface of Lactococcus lactis. It is known to take part in the proteolysis of many secreted recombinant proteins, and the mutation of htrA can lead to the complete stabilization of recombinant proteins. In this work, we have shown that htrA mutation also leads to significant reduction of the efficiency of recombinant-protein secretion. We also show that the level of HtrA can be lowered by the suppression of the acid tolerance response (ATR) in L. lactis. Instead of using an L. lactis htrA mutant, the reduction of the HtrA level in wild-type recombinant cultures of L. lactis by ATR suppression may serve as a better strategy for the production of secreted recombinant proteins.

* Corresponding author. Mailing address: Department of Biotechnology, Indian Institute of Technology—Madras, Chennai, India 600036. Phone: 91 44 22574108. Fax: 91 44 22574102. E-mail: guhanj{at}iitm.ac.in

{triangledown} Published ahead of print on 3 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7442-7446, Vol. 74, No. 23
0099-2240/08/$08.00+0     doi:10.1128/AEM.00638-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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