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Applied and Environmental Microbiology, December 2008, p. 7620-7628, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.00972-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Distribution of Crenarchaeota Representatives in Terrestrial Hot Springs of Russia and Iceland {triangledown}

Anna A. Perevalova,1* Tatiana V. Kolganova,2 Nils-Kåre Birkeland,3 Christa Schleper,3 Elizaveta A. Bonch-Osmolovskaya,1 and Alexander V. Lebedinsky1

Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia,1 Bioengineering Center, Russian Academy of Sciences, Prospekt 60-letiya Oktyabrya, 7/1, 117312 Moscow, Russia,2 Department of Biology and Centre for Geobiology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway3

Received 29 April 2008/ Accepted 6 October 2008

Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial hot springs with moderate temperatures (55 to 70°C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, "Fervidococcus fontis," which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.

* Corresponding author. Mailing address: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia. Phone: 7 (499) 135-4458. Fax: 7 (499) 135-6530. E-mail: annprv{at}gmail.com

{triangledown} Published ahead of print on 10 October 2008.

Applied and Environmental Microbiology, December 2008, p. 7620-7628, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.00972-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kublanov, I. V., Perevalova, A. A., Slobodkina, G. B., Lebedinsky, A. V., Bidzhieva, S. K., Kolganova, T. V., Kaliberda, E. N., Rumsh, L. D., Haertle, T., Bonch-Osmolovskaya, E. A. (2009). Biodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia). Appl. Environ. Microbiol. 75: 286-291 [Abstract] [Full Text]  


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