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Applied and Environmental Microbiology, December 2008, p. 7779-7789, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01412-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Control of Lipid Accumulation in the Yeast Yarrowia lipolytica{triangledown}

Athanasios Beopoulos,1,2,3 Zuzana Mrozova,1,2,4 France Thevenieau,1,{dagger} Marie-Thérèse Le Dall,1 Ivan Hapala,4 Seraphim Papanikolaou,3 Thierry Chardot,2 and Jean-Marc Nicaud1*

Laboratoire de Microbiologie et Génétique Moléculaire, INRA, UMR1238, CNRS, UMR2585, AgroParisTech, Centre de Biotechnologie Agro-Industrielle, BP 01, 78850 Thiverval-Grignon, France,1 Laboratoire de Chimie Biologique, INRA, UMR206, AgroParisTech, Centre de Biotechnologie Agro-Industrielle, 78850 Thiverval-Grignon, France,2 Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Technology, Iera Odos 75, 11855 Athens, Greece,3 Institute of Animal Biochemistry andproc Genetics, Slovak Academy of Sciences, Moyzesova 61, Ivanka pri Dunaji 900 28, Slovakia4

Received 24 June 2008/ Accepted 17 October 2008

A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolytica is oriented toward the glycerol pathway. To redirect carbon flux toward lipid synthesis, the GUT2 gene, which codes for the glycerol-3-phosphate dehydrogenase isomer, was deleted in Y. lipolytica in this study. This {Delta}gut2 mutant strain demonstrated a threefold increase in lipid accumulation compared to the wild-type strain. However, mobilization of lipid reserves occurred after the exit from the exponential phase due to β-oxidation. Y. lipolytica contains six acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX6 genes, that catalyze the limiting step of peroxisomal β-oxidation. Additional deletion of the POX1 to POX6 genes in the {Delta}gut2 strain led to a fourfold increase in lipid content. The lipid composition of all of the strains tested demonstrated high proportions of FFA. The size and number of the lipid bodies in these strains were shown to be dependent on the lipid composition and accumulation ratio.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Génétique Moléculaire, AgroParisTech, Centre de Biotechnologie Agro-Industrielle, INRA Centre de Grignon, BP 01, 78850 Thiverval-Grignon, France. Phone: 33 01 30 81 54 50. Fax: 33 01 30 81 54 57. E-mail: jean-marc.nicaud{at}grignon.inra.fr

{triangledown} Published ahead of print on 24 October 2008.

{dagger} Present address: Oxyrane UK Ltd., Greenheys House, Manchester Science Park, 10 Pencroft Way, Manchester M15 6JJ, United Kingdom.

Applied and Environmental Microbiology, December 2008, p. 7779-7789, Vol. 74, No. 24
0099-2240/08/$08.00+0     doi:10.1128/AEM.01412-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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