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Applied and Environmental Microbiology, February 2008, p. 889-893, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.00684-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluations of Different Hypervariable Regions of Archaeal 16S rRNA Genes in Profiling of Methanogens by Archaea-Specific PCR and Denaturing Gradient Gel Electrophoresis{triangledown}

Zhongtang Yu,1* Rubén García-González,2 Floyd L. Schanbacher,1 and Mark Morrison3

The MAPLE Research Initiative, Department of Animal Sciences, The Ohio State University, Columbus, Ohio 43210,1 Departamento de Producción Animal, Universidad de León, León 24071, Spain,2 CSIRO Livestock Industries, St. Lucia, Australia3

Received 26 March 2007/ Accepted 30 October 2007

Different hypervariable (V) regions of the archaeal 16S rRNA gene (rrs) were compared systematically to establish a preferred V region(s) for use in Archaea-specific PCR-denaturing gradient gel electrophoresis (DGGE). The PCR products of the V3 region produced the most informative DGGE profiles and permitted identification of common methanogens from rumen samples from sheep. This study also showed that different methanogens might be detected when different V regions are targeted by PCR-DGGE. Dietary fat appeared to transiently stimulate Methanosphaera stadtmanae but inhibit Methanobrevibacter sp. strain AbM4 in rumen samples.

* Corresponding author. Mailing address: Department of Animal Sciences, The Ohio State University, 2029 Fyffe Court, Columbus, OH 43210. Phone: (614) 292-3057. Fax: (614) 292-2929. E-mail: yu.226{at}osu.edu

{triangledown} Published ahead of print on 14 December 2007.

Applied and Environmental Microbiology, February 2008, p. 889-893, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.00684-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Karnati, S. K. R., Yu, Z., Firkins, J. L. (2009). Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. II. Interaction of treatment and presence of protozoa on prokaryotic communities. J DAIRY SCI 92: 3861-3873 [Abstract] [Full Text]  


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