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Applied and Environmental Microbiology, February 2008, p. 912-915, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01655-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of the Pneumococcal Serotype 14 Polysaccharide in Lactococcus lactis Requires Lactococcal epsABC Regulatory Genes{triangledown} ,{dagger}

Masja N. Nierop Groot,{ddagger} Jeroen Godefrooij, and Michiel Kleerebezem*

TI Food and Nutrition, NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands

Received 19 July 2007/ Accepted 2 December 2007

The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL14) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCDB40). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth.

* Corresponding author. Mailing address: NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-318-659629. Fax: 31-318-650400. E-mail: Michiel.Kleerebezem{at}nizo.nl

{triangledown} Published ahead of print on 14 December 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: TI Food and Nutrition, Bornsesteeg 59, P.O. Box 17, 6700 AA Wageningen, The Netherlands.

Applied and Environmental Microbiology, February 2008, p. 912-915, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01655-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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