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Applied and Environmental Microbiology, January 2009, p. 101-107, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01749-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cloning of the Trichoderma reesei cDNA Encoding a Glucuronan Lyase Belonging to a Novel Polysaccharide Lyase Family{triangledown} ,{dagger}

Naotake Konno,1 Kiyohiko Igarashi,1 Naoto Habu,2 Masahiro Samejima,1 and Akira Isogai1*

Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan,1 Department of Bioproductive Science, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan2

Received 30 July 2008/ Accepted 21 October 2008

The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on β-(1->4)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris. The cDNA of TrGL includes a 777-bp open reading frame encoding a 20-amino-acid signal peptide and the 238-amino-acid mature protein. The amino acid sequence showed no similarity to the amino acid sequences of previously described functional proteins, indicating that the enzyme should be classified in a novel polysaccharide lyase (PL) family. Recombinant TrGL catalyzed depolymerization of cellouronate endolytically by β-elimination and was highly specific for cellouronate. The enzyme was most active at pH 6.5 and 50°C, and its activity and thermostability increased in the presence of Ca2+, suggesting that its calcium dependence is similar to that of other PLs, such as pectate lyases.

* Corresponding author. Mailing address: 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81-3-5841-5538. Fax: 81-3-5841-5269. E-mail: aisogai{at}mail.ecc.u-tokyo.ac.jp

{triangledown} Published ahead of print on 31 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2009, p. 101-107, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01749-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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