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Applied and Environmental Microbiology, January 2009, p. 257-260, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01400-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Novel Cold-Adapted Alkaline Lipase from an Intertidal Flat Metagenome and Proposal for a New Family of Bacterial Lipases {triangledown} ,{dagger}

Eun-Young Kim,{ddagger} Ki-Hoon Oh,{ddagger} Mi-Hwa Lee, Chul-Hyung Kang, Tae-Kwang Oh, and Jung-Hoon Yoon*

Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejon, Republic of Korea

Received 23 June 2008/ Accepted 9 October 2008

A new lipase, LipEH166, isolated from an intertidal flat metagenome, showed no amino acid similarity to any known lipolytic enzyme except in the consensus region. This suggested that LipEH166 and its homologues belong to a new family of lipolytic enzymes. Partial characterization indicated that LipEH166 is a novel cold-adapted alkaline lipase.

* Corresponding author. Mailing address: Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yuseong, Daejon 305-333, Republic of Korea. Phone: 82 42 860 4276. Fax: 82 42 879 8595. E-mail: jhyoon{at}kribb.re.kr

{triangledown} Published ahead of print on 17 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} E.-Y.K. and K.-H.O. contributed equally to this work.

Applied and Environmental Microbiology, January 2009, p. 257-260, Vol. 75, No. 1
0099-2240/09/$08.00+0     doi:10.1128/AEM.01400-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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