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Applied and Environmental Microbiology, May 2009, p. 3348-3351, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00560-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Microbial Diversity of Septic Tank Effluent and a Soil Biomat{triangledown} ,{dagger}

Jill Tomaras, Jason W. Sahl, Robert L. Siegrist, and John R. Spear*

Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401

Received 7 March 2008/ Accepted 6 March 2009

Microbial diversity of septic tank effluent (STE) and the biomat that is formed as a result of STE infiltration on soil were characterized by 16S rRNA gene sequence analysis. Results indicate that microbial communities are different within control soil, STE, and the biomat and that microbes found in STE are not found in the biomat. The development of a stable soil biomat appears to provide the best on-site water treatment or protection for subsequent groundwater interactions of STE.

* Corresponding author. Mailing address: Environmental Science and Engineering Division, Colorado School of Mines, Golden, CO 80401. Phone: (303) 273-3497. Fax: (303) 273-3413. E-mail: jspear{at}mines.edu

{triangledown} Published ahead of print on 20 March 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 3348-3351, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00560-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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