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Applied and Environmental Microbiology, June 2009, p. 4197-4201, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.00156-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Haloprotease CPI Produced by the Moderately Halophilic Bacterium Pseudoalteromonas ruthenica Is Secreted by the Type II Secretion Pathway{triangledown} ,{dagger}

Cristina Sánchez-Porro,1,2 Encarnación Mellado,1 Anthony P. Pugsley,2 Olivera Francetic,2 and Antonio Ventosa1*

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain,1 Institut Pasteur, Unité de Génétique Moléculaire, and CNRS URA2172, Paris, France2

Received 22 January 2009/ Accepted 11 April 2009

The gene (cpo) encoding the extracellular protease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica CP76 was cloned, and its nucleotide sequence was analyzed. The cpo gene encodes a 733-residue protein showing sequence similarity to metalloproteases of the M4 family. The type II secretion apparatus was shown to be responsible for secretion of the haloprotease CPI.


* Corresponding author. Mailing address: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain. Phone: 34 95 455 6765. Fax: 34 95 462 8162. E-mail: ventosa{at}us.es

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.


Applied and Environmental Microbiology, June 2009, p. 4197-4201, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.00156-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.