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Applied and Environmental Microbiology, June 2009, p. 4206-4210, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02906-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Promoters for Efficient Gene Expression in Magnetospirillum gryphiswaldense{triangledown} ,{dagger}

Claus Lang, Anna Pollithy, and Dirk Schüler*

Ludwig-Maximillians-Universität München, Department Biologie I, Bereich Mikrobiologie, Großhadernerstrasse 4, 82152 Planegg-Martinsried, Germany

Received 20 December 2008/ Accepted 10 April 2009

To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli Plac promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal PmamDC promoter. We demonstrate that this promoter can be used for the expression of modified magnetosome proteins to generate "antibody-binding" magnetosomes.

* Corresponding author. Mailing address: Ludwig-Maximillians-Universität München, Department Biologie I, Bereich Mikrobiologie, Großhadernerstr. 4, 82152 Planegg-Martinsried, Germany. Phone: 49 89 21 80-74502. Fax: 49 89 21 80-74515. E-mail: dirk.schueler{at}lrz.uni-muenchen.de

{triangledown} Published ahead of print on 24 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2009, p. 4206-4210, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02906-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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