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Applied and Environmental Microbiology, July 2009, p. 4620-4623, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00582-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

[FeFe] Hydrogenase Genetic Diversity Provides Insight into Molecular Adaptation in a Saline Microbial Mat Community{triangledown} ,{dagger}

Eric S. Boyd,1 John R. Spear,2 and John W. Peters1*

Department of Chemistry and Biochemistry and Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717,1 Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 804012

Received 10 March 2009/ Accepted 29 April 2009

Degenerate primers for the [FeFe] hydrogenase (hydA) were developed and used in PCRs to examine hydA in microbial mats that inhabit saltern evaporative ponds in Guerrero Negro (GN), Mexico. A diversity of deduced HydA was discovered that revealed unique variants, which may reflect adaptation to the environmental conditions present in GN.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, 103 Chemistry Research Building, Montana State University, Bozeman, MT 59717. Phone: (406) 994-7211. Fax: (406) 994-5407. E-mail: John.peters{at}chemistry.montana.edu

{triangledown} Published ahead of print on 8 May 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2009, p. 4620-4623, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00582-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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