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Applied and Environmental Microbiology, July 2009, p. 4630-4632, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02872-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Influence of Culture Conditions and Medium Composition on the Production of Cellulose by Shiga Toxin-Producing Escherichia coli Cells {triangledown}

Byong Kwon Yoo and Jinru Chen*

Department of Food Science and Technology, The University of Georgia, 1109 Experiment St., Griffin, Georgia 30223-1797

Received 17 December 2008/ Accepted 19 April 2009

Culture conditions favoring cellulose production by Shiga toxin-producing Escherichia coli included a 28°C incubation temperature, an aerobic atmosphere, and the presence of 2% ethanol in Luria-Bertani no-salt agar with pH 6.0 and a water activity of 0.99. These findings will assist in formulating microbiological media useful for cellulose and biofilm research.

* Corresponding author. Mailing address: Department of Food Science and Technology, The University of Georgia, 1109 Experiment St., Griffin, GA 30223-1797. Phone: (770) 412-4738. Fax: (770) 412-4748. E-mail: jchen{at}uga.edu

{triangledown} Published ahead of print on 1 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4630-4632, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02872-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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