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Applied and Environmental Microbiology, July 2009, p. 4653-4656, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00225-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Selected Pseudomonas putida Strains Able To Grow in the Presence of High Butanol Concentrations{triangledown} ,{dagger}

Jana Rühl,1 Andreas Schmid,1,2* and Lars Mathias Blank1,2

Laboratory of Chemical Biotechnology, Technische Universität Dortmund, Emil-Figge-Strasse 66, D-44221 Dortmund, Germany,1 ISAS—Institute for Analytical Sciences, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, Germany2

Received 30 January 2009/ Accepted 27 April 2009

Adapted Pseudomonas putida strains grew in the presence of up to 6% (vol/vol) butanol, the highest reported butanol concentration tolerated by a microbe. P. putida might be an alternative host for biobutanol production, overcoming the primary limitation of currently used strains—insufficient product titers due to low butanol tolerance.

* Corresponding author. Mailing address: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44221 Dortmund, Germany. Phone: 49 231 755 7380. Fax: 49 231 755 7382. E-mail: andreas.schmid{at}bci.tu-dortmund.de

{triangledown} Published ahead of print on 1 May 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2009, p. 4653-4656, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00225-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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