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Applied and Environmental Microbiology, July 2009, p. 4821-4828, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00342-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Syntrophic Degradation of Cadaverine by a Defined Methanogenic Coculture{triangledown}

Julia Roeder and Bernhard Schink*

Fachbereich Biologie, University of Konstanz, D-78457 Constance, Germany

Received 11 February 2009/ Accepted 13 May 2009

A novel, strictly anaerobic, cadaverine-oxidizing, defined coculture was isolated from an anoxic freshwater sediment sample. The coculture oxidized cadaverine (1,5-diaminopentane) with sulfate as the electron acceptor. The sulfate-reducing partner could be replaced by a hydrogenotrophic methanogenic partner. The defined coculture fermented cadaverine to acetate, butyrate, and glutarate plus sulfide or methane. The key enzymes involved in cadaverine degradation were identified in cell extracts. A pathway of cadaverine fermentation via 5-aminovaleraldehyde and crotonyl-coenzyme A with subsequent dismutation to acetate and butyrate is suggested. Comparative 16S rRNA gene analysis indicated that the fermenting part of the coculture belongs to the subphylum Firmicutes but that this part is distant from any described genus. The closest known relative was Clostridium aminobutyricum, with 95% similarity.

* Corresponding author. Mailing address: Fachbereich Biologie, University of Konstanz, D-78457 Constance, Germany. Phone: 49 7531 882140. Fax: 49 7531 884047. E-mail: Bernhard.Schink{at}uni-konstanz.de

{triangledown} Published ahead of print on 22 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4821-4828, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00342-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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