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Applied and Environmental Microbiology, July 2009, p. 4892-4896, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.02835-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cultivation of Methanogens under Low-Hydrogen Conditions by Using the Coculture Method{triangledown} ,{dagger}

Sanae Sakai,1,2 Hiroyuki Imachi,2* Yuji Sekiguchi,3 I-Cheng Tseng,4 Akiyoshi Ohashi,1,5 Hideki Harada,1,6 and Yoichi Kamagata1,7

Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan,1 Subsurface Geobiology Advanced Research (SUGAR) Team, Institute of Biogeosciences, Japan Agency for Marine-Earth Science & Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan,2 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan,3 Department of Biology, National Cheng Kung University, Tainan 701, Taiwan,4 Department of Social and Environmental Engineering, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8527, Japan,5 Department of Civil and Environmental Engineering, Tohoku University, Sendai 980-8579, Japan,6 Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Hokkaido 062-8517, Japan7

Received 15 December 2008/ Accepted 14 May 2009

We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within the order Methanomicrobiales.

* Corresponding author. Mailing address: Subsurface Geobiology Advanced Research (SUGAR) Team, Institute of Biogeosciences, Japan Agency for Marine-Earth Science & Technology (JAMSTEC), 2-15 Natsushima-Cho, Yokosuka, Kanagawa 237-0061, Japan. Phone: 81-46-867-9709. Fax: 81-46-867-9715. E-mail: imachi{at}jamstec.go.jp

{triangledown} Published ahead of print on 22 May 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2009, p. 4892-4896, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.02835-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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