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Applied and Environmental Microbiology, January 2009, p. 534-537, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01706-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach{triangledown} ,{dagger}

Sandrine Demanèche,1 Laurent Philippot,2,3 Maude M. David,1 Elisabeth Navarro,1,4 Timothy M. Vogel,1* and Pascal Simonet1

Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France,1 INRA, UMR 1229, F-21000 Dijon, France,2 University of Burgundy, UMR 1229, F-21000 Dijon, France,3 Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR 113 IRD, CIRAD, AGRO Montpellier, INRA, Université Montpellier 2, TA A-82/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France4

Received 24 July 2008/ Accepted 3 November 2008

We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

* Corresponding author. Mailing address: Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France. Phone: (33) 4 72 18 65 14. Fax: (33) 4 78 43 37 17. E-mail: tvogel{at}ec-lyon.fr

{triangledown} Published ahead of print on 14 November 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2009, p. 534-537, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01706-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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