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Applied and Environmental Microbiology, January 2009, p. 542-546, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01816-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Silencing of Six Hydrophobins in Cladosporium fulvum: Complexities of Simultaneously Targeting Multiple Genes{triangledown}

Hélène Lacroix{dagger} and Pietro D. Spanu*

Department of Life Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom

Received 6 August 2008/ Accepted 11 November 2008

In this study, we have constructed and expressed inverted repeat chimeras from the first exons of the six known hydrophobins of the fungus Cladosporium fulvum, the causal agent of tomato leaf mold. We used quantitative PCR to measure specifically the expression levels of the hydrophobins. The targeted genes are silenced to different degrees, but we also detected clear changes in the expression levels of nontargeted genes. This work highlights the difficulties that are likely to be encountered when attempting to silence more than one gene in a multigene family.

* Corresponding author. Mailing address: Department of Life Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom. Phone: 44-20-75945384. Fax: 44-20-75842056. E-mail: p.spanu{at}imperial.ac.uk

{triangledown} Published ahead of print on 14 November 2008.

{dagger} Present address: Laboratoire Stress, Défenses et Reproduction des Plantes, Université de Reims, UFR Sciences Exactes et Naturelles, Moulin de la Housse bât. 18, BP 1039, 51687 Reims Cedex, France.

Applied and Environmental Microbiology, January 2009, p. 542-546, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01816-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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