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Applied and Environmental Microbiology, January 2009, p. 547-550, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01665-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Wolbachia Bacteria in Multiple Organs and Feces of the Triatomine Insect Rhodnius pallescens (Hemiptera, Reduviidae){triangledown}

C. I. Espino,1 T. Gómez,1 G. González,1,2 M. F. Brazil do Santos,2 J. Solano,2 O. Sousa,1 N. Moreno,1 D. Windsor,3 A. Ying,1 S. Vilchez,2 and A. Osuna2*

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Panamá, República de Panamá,1 Grupo de Bioquímica y Parasitología Molecular, Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, 18071 Granada, Spain,2 Smithsonian Tropical Research Institute, Apdo. 0843-03092, Panamá, República de Panamá3

Received 18 July 2008/ Accepted 12 November 2008

At least two types of Wolbachia bacteria were detected in wild and insectarium-raised Rhodnius pallescens, a natural vector of Trypanosoma cruzi and Trypanosoma rangeli. Wolbachia was detected in all the organs and tissues studied and in the feces, and this provided a methodological advantage for determining the presence of this endosymbiont in this host, obviating the need to kill the specimens. The occurrence of trypanosomatids in wild individuals was also studied.

* Corresponding author. Mailing address: Institute of Biotechnology, University of Granada, Campus Universitario Fuentenueva, E-18071 Granada, Spain. Phone: 34-958244163. Fax: 34-958243174. E-mail: aosuna{at}ugr.es

{triangledown} Published ahead of print on 21 November 2008.

Applied and Environmental Microbiology, January 2009, p. 547-550, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01665-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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