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Applied and Environmental Microbiology, February 2009, p. 603-607, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01874-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

TatABC Overexpression Improves Corynebacterium glutamicum Tat-Dependent Protein Secretion{triangledown}

Yoshimi Kikuchi,1,{dagger}* Hiroshi Itaya,1,{dagger} Masayo Date,1 Kazuhiko Matsui,1 and Long-Fei Wu2

Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan,1 Laboratoire de Chimie Bactérienne, UPR9043, Institut de Biologie Structurale et Microbiologie, CNRS, F-13402 Marseille, France2

Received 13 August 2008/ Accepted 2 December 2008

The twin-arginine translocation (Tat) pathway in Corynebacterium glutamicum has been described previously. The minimal functional Tat system in C. glutamicum required TatA and TatC but did not require TatB, although this component was required for maximal efficiency of Tat-dependent secretion. We previously demonstrated that Chryseobacterium proteolyticum pro-protein glutaminase (pro-PG) and Streptomyces mobaraensis pro-transglutaminase (pro-TG) could be secreted via the Tat pathway in C. glutamicum. Here we report that the amounts of pro-PG secreted were more than threefold larger when TatC or TatAC was overexpressed, and there was a further threefold increase when TatABC was overexpressed. These results show that the amount of TatC protein is the first bottleneck and the amount of TatB protein is the second bottleneck in Tat-dependent protein secretion in C. glutamicum. In addition, the amount of pro-TG that accumulated via the Tat pathway when TatABC was overexpressed with the TorA signal peptide in C. glutamicum was larger than the amount that accumulated via the Sec pathway. We concluded that TatABC overexpression improves Tat-dependent pro-PG and pro-TG secretion in C. glutamicum.

* Corresponding author. Mailing address: Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan. Phone: 81-44-244-7123. Fax: 81-44-222-0129. E-mail: yoshimi_kikuchi{at}ajinomoto.com

{triangledown} Published ahead of print on 12 December 2008.

{dagger} Y.K. and H.I. contributed equally to this work.

Applied and Environmental Microbiology, February 2009, p. 603-607, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01874-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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