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Applied and Environmental Microbiology, February 2009, p. 608-617, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01183-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea{triangledown}

Laura Bruno, Daniela Billi, Simona Bellezza, and Patrizia Albertano*

Department of Biology, University of Rome Tor Vergata, 00173 Rome, Italy

Received 28 May 2008/ Accepted 21 November 2008

Six Leptolyngbya strains, isolated from the archaeological surfaces of hypogean sites, were phenotypically and genetically characterized by light and electron microscopy and 16S rRNA gene and 16S-23S internally transcribed spacer (ITS) sequencing. Three phycoerythrin-rich (red) and three phycocyanin-rich (green) isolates were assigned to different operational taxonomic units (OTUs). Among the green isolates, one strain showed an OTU intraspecific variation due to differences in the ITS sequences and genomic polymorphism. Within the ITS sequence, variable regions, conserved domains and tRNAIle and tRNAAla genes showed high sequence identity among the phylotypes. Together, these data indicated a relatedness of the six strains to other Leptolyngbya from subaerophytic and geothermal environments and allowed the definition of novel Leptolyngbya OTUs.

* Corresponding author. Mailing address: University of Rome Tor Vergata, Department of Biology, via della Ricerca Scientifica, 00173 Rome, Italy. Phone: 39 06 7259 4332. Fax: 39 06 2023500. E-mail: albertano{at}uniroma2.it

{triangledown} Published ahead of print on 1 December 2008.

Applied and Environmental Microbiology, February 2009, p. 608-617, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01183-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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