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Applied and Environmental Microbiology, February 2009, p. 687-694, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01506-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Bioreduction with Efficient Recycling of NADPH by Coupled Permeabilized Microorganisms{triangledown}

Wei Zhang,1,2 Kevin O'Connor,3 Daniel I. C. Wang,1,4 and Zhi Li1,2*

Singapore-MIT Alliance, National University of Singapore, Singapore,1 Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore,2 Department of Industrial Microbiology, University College Dublin, Dublin, Ireland,3 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts4

Received 3 July 2008/ Accepted 24 November 2008

The glucose dehydrogenase (GDH) from Bacillus subtilis BGSC 1A1 was cloned and functionally expressed in Escherichia coli BL21(pGDH1) and XL-1 Blue(pGDH1). Controlled permeabilization of recombinant E. coli BL21 and XL-1 Blue with EDTA-toluene under optimized conditions resulted in permeabilized cells with specific activities of 61 and 14 U/g (dry weight) of cells, respectively, for the conversion of NADP+ to NADPH upon oxidation of glucose. The permeabilized recombinant strains were more active than permeabilized B. subtilis BGSC 1A1, did not exhibit NADPH/NADH oxidase activity, and were useful for regeneration of both NADH and NADPH. Coupling of permeabilized cells of Bacillus pumilus Phe-C3 containing an NADPH-dependent ketoreductase and an E. coli recombinant expressing GDH as a novel biocatalytic system allowed enantioselective reduction of ethyl 3-keto-4,4,4-trifluorobutyrate with efficient recycling of NADPH; a total turnover number (TTN) of 4,200 mol/mol was obtained by using E. coli BL21(pGDH1) as the cofactor-regenerating microorganism with initial addition of 0.005 mM NADP+. The high TTN obtained is in the practical range for producing fine chemicals. Long-term stability of the permeabilized cell couple and a higher product concentration were demonstrated by 68 h of bioreduction of ethyl 3-keto-4,4,4-trifluorobutyrate with addition of 0.005 mM NADP+ three times; 50.5 mM (R)-ethyl 3-hydroxy-4,4,4-trifluorobutyrate was obtained with 95% enantiomeric excess, 84% conversion, and an overall TTN of 3,400 mol/mol. Our method results in practical synthesis of (R)-ethyl 3-hydroxy-4,4,4-trifluorobutyrate, and the principle described here is generally applicable to other microbial reductions with cofactor recycling.

* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576. Phone: 65-65168416. Fax: 65-67791936. E-mail: chelz{at}nus.edu.sg

{triangledown} Published ahead of print on 1 December 2008.

Applied and Environmental Microbiology, February 2009, p. 687-694, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01506-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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